Unknown

yard Negative Unknown Upon being given a bacteriuml elaboration containing two bacterium, I was assigned the task of keep a pop out the several(prenominal) bacteria. The two bacteria of the mixture were iodin one thousandme unequivocal and one gigabyte negative. The experiment has two split and this is the part of isolating the yard-negative bacteria. The technique used was called streaking for closing reach through in which the bacteria were streaked thinly on food for thought agar in different directions in a normal that left individual colonies on the last-place growth streak. These individual colonies could be identified by their individual characteristics such as color and shape and distinguished from one another. This was not tardily with this nicety because both bacteria grew in colonies that were small, white, and shaped similar. To sieve these bacteria I had to first gram stain the bacterial mixture to see if I had both types of bacteria and so I us ed the following travel to perform the gram stain. 1. The bacteria are discolourationed on a slide and allowed to dry out. 2. The bacteria is heat fixed to the slide 3. watch crystal violet pigment is added to the smear for 1 minute 4. purify the pigment hit with distilled peeing 5. Apply Grams Iodine for 1 minute 6. Wash iodine off with distilled water 7.

Decolorize the smear with 95% ethyl soaker beverage hurtle by drop until it runs off conk 8. Wash off alcohol with distilled water 9. Counterstain with Safranin for 45 seconds 10. Wash off Safranin with distilled water 11. Blot dry with Bib ulous paper Following this test, I found! that I had a lot more gram negative bacteria than the gram positive and so I could easily lend one colony of my streaked market-gardening and have a high chance of growing a pure culture from one of the isolated colonies. So, with this method, I grew a pure culture of the gram negative bacteria that I would use in my tests to determine its identity. I ran several tests on the bacteria stark(a) with the Gram stain that proved that the bacteria were gram negative....If you compulsion to get a full essay, couch it on our website: OrderCustomPaper.com

If you want to get a full essay, visit our page: write my paper